human osteoblast cell line nhost cells  (Cambrex)

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Characterization and modeling of additively manufactured Ti-6Al-4V alloy with modified surfaces for medical applications

doi: 10.3389/fbioe.2025.1526873

Figure Lengend Snippet: (a) NHOst viability (relative to the positive control) after 24 h of cultivation (white columns), showing the extent of cell adhesion and after 72 h of cultivation (gray columns), showing the extent of cell proliferation, examined on MTi, SBTi and AMTi discs, non-coated (normal bars) and coated with PEM coating (hatched bars) (* designates values that are significantly different, p < 0.05). SEM images of NHOst cultured for 24 h (upper row) and 72 h (bottom row) on Ti-6Al-4V discs with increasing roughness: (b, e) MTi, (c, f) SBTi, and (d, g) AMTi. Magnification 1,200×.

Article Snippet: Cells viability and proliferation on the uncoated and PEM-coated Ti-6Al-4V specimen (machined, sand-blasted and additively manufactured) with different roughness levels were investigated with 4 cell types - L929 fibroblasts (cell lines service GmbH, Eppelheim, Germany, Cat. No.: 400260–817), MC3T3 preosteoblasts (American Type Culture Collection, Virginia, United States, Cat. No.: CRL-2593), human dental pulp stem cells (hDPSCs), and human osteoblasts (NHOst) (Lonza Group AG, Basel, Switzerland, Cat No.: CC-2538) based on previously described protocols ( ; ).

Techniques: Positive Control, Cell Culture

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Biphasic bone substitutes coated with PLGA incorporating therapeutic ions Sr 2+ and Mg 2+ : cytotoxicity cascade and in vivo response of immune and bone regeneration

doi: 10.3389/fbioe.2024.1408702

Figure Lengend Snippet: An overview of the implantation areas in all study groups at day 10 post-implantation and the tissue reactions to the BBS granules. BBS = granules of the biphasic bone substitute, LB = local bone, CT = connective tissue, red stars = newly formed bone matrix, red arrows = blood vessels, white arrows = new material-related bone, black arrows = macrophages, yellow arrowheads = multinucleated giant cells, blue arrows = osteoblasts (Movat Pentachrome-stainings, (A1–C1) , ×100 magnification, scale bar = 50 μm; (A2–C2) , ×200 magnification, scale bar = 20 µm).

Article Snippet: Primary normal human osteoblasts (NHOst; Lonza) were cultured in OGM (OBM supplemented with OGM Single Quots; Lonza) and grown as monolayer cultures in T75 flasks (Greiner Bio-One).

Techniques:

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Biphasic bone substitutes coated with PLGA incorporating therapeutic ions Sr 2+ and Mg 2+ : cytotoxicity cascade and in vivo response of immune and bone regeneration

doi: 10.3389/fbioe.2024.1408702

Figure Lengend Snippet: An overview of the implantation areas in all study groups at day 30 post implantation and the tissue reactions to the bone substitute granules. BBS = granules of the biphasic bone substitute, LB = local bone, CT = connective tissue, red stars = newly formed bone matrix, red arrows = blood vessels, black arrows = macrophages, yellow arrowheads = multinucleated giant cells, blue arrows = osteoblasts (Movat Pentachrome-stainings, (A1–C1) , ×100 magnification, scale bar = 50 μm; (A2–C2) , ×200 magnification, scale bar = 20 µm).

Article Snippet: Primary normal human osteoblasts (NHOst; Lonza) were cultured in OGM (OBM supplemented with OGM Single Quots; Lonza) and grown as monolayer cultures in T75 flasks (Greiner Bio-One).

Techniques:

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Biphasic bone substitutes coated with PLGA incorporating therapeutic ions Sr 2+ and Mg 2+ : cytotoxicity cascade and in vivo response of immune and bone regeneration

doi: 10.3389/fbioe.2024.1408702

Figure Lengend Snippet: An overview of the implantation areas in all groups at day 90 post implantation and the tissue reaction to the bone substitutes granules. BBS = granules of the biphasic bone substitute, LB = local bone, CT = connective tissue, red stars = newly formed bone matrix, red arrows = blood vessels, black arrows = macrophages, yellow arrowheads = multinucleated giant cells, blue arrows = osteoblasts (Movat Pentachrome-stainings, (A1–C1) , ×100 magnification, scale bar = 50 μm; (A2–C2) , ×200 magnification, scale bar = 20 µm).

Article Snippet: Primary normal human osteoblasts (NHOst; Lonza) were cultured in OGM (OBM supplemented with OGM Single Quots; Lonza) and grown as monolayer cultures in T75 flasks (Greiner Bio-One).

Techniques:

Journal: Tissue engineering

Article Title: Noninvasive in Situ Evaluation of Osteogenic Differentiation by Time-Resolved Laser-Induced Fluorescence Spectroscopy

doi: 10.1089/107632704323061771

Figure Lengend Snippet: PLA cells possess osteogenic capacity. (A) Human PLA cells induced with osteogenic medium (Induced PLA) for 3 weeks were stained for AP activity (AP, dark gray) and matrix mineralization (VK, black staining). A human osteoblast cell line (NHOst) served as a positive control. Original magnification, ×200. (B) PLA cells cultured in OM for 3 weeks (Induced PLA) were analyzed by RT-PCR for the expression of osteocalcin (OC), osteopontin (OP), osteonectin (ON), core-binding factor 1 (Cbfa-1), and AP.

Article Snippet: A human osteoblast cell line (NHOst cells; Cambrex Bio Science Walkersville, Walkersville, MD) served as a positive control and PLA cells cultured in CM were a negative control.

Techniques: Staining, Activity Assay, Positive Control, Cell Culture, Reverse Transcription Polymerase Chain Reaction, Expressing, Binding Assay